Stalking Premium Human VLDL?
KALEN Human VLDL (d= <1.006 g/mL) is isolated from human plasma by ultracentrifugation. The purified VLDL is ultrafiltered through a membrane and packaged aseptically under nitrogen. The product is provided in a solution with 154 mM NaCl and 0.34 uM EDTA, at pH 7.2. The protein concentration is 1.0 mg/mL. The purity of the product is assessed by agarose gel electrophoresis.
| Catalog Number | Size | Price USD |
| 770100-7 | 1 mg | $155.00 |
| 770100-4 | 5 mg | $550.00 |
| 770100-1 | 10 mg | $945.00 |
Kalen now offers DiI labeled Very Low Density Lipoprotein for use as a probe in lipid metabolism uptake studies. DiI (3,3'-dioctadecylindocarbocyanine) is a fluorescent dye which permeates the lipid portion of the lipoprotein particle. DiI is a carbocyanine dye that has been used extensively for labelling membranes. It is only slightly fluorescent in aqueous solutions, but highly fluorescent in a lipid environment. DiI has been excited at 488nm or 514nm and the emission is detected at 565 nm. These probes can be detected with a fluorescent microscope, fluorescent plate reader, or flow cytometer.
| Probe | Catalog Number |
Size | Price USD |
| DiI-VLDL | 770130-9 | 0.5 mg | $360.00 |
Very Low Density Lipoprotein (VLDL) is a major transporter of endogenous lipids (cholesterol and its esters, triglycerides and phospholipids). The particles, which range in size from 30 to 80 nm in diameter, are first assembled from apolipoproteins and cholesterol in the liver (see figure at right). Subsequently, maturation of the particles occurs in the bloodstream where additional apolipoproteins and lipids are incorporated.
Lipoprotein lipase mediates the removal of triglycerides from the VLDL particles resulting in the formation of intermediate density lipoprotein (IDL) particles with densities ranging from 1.006 to 1.019 g/mL. Maturation of the IDL particles eventually leads to the formation of low density lipoprotein (LDL) particles having a density of 1.019 - 1.063 g/mL.
