Low Density Lipoprotein

KALEN Human LDL^TM

KALEN Human LDL (d=1.019 – 1.063 g/mL) is isolated from human plasma by ultracentrifugation. The purified LDL is ultrafiltered and packaged aseptically under nitrogen. The lipoprotein is provided in a solution with 154 mM NaCl and 0.34 uM EDTA, pH 7.2, at a protein concentration of 5 mg/mL. Product purity is assessed by agarose gel electrophoresis.

KALEN Human LDL is manufactured with our proprietary manufacturing protocols and stringent quality controls. To ensure against unwanted oxidation, each lot of the product is tested using the TBARS assay (see box to the right). Typical results for TBARS assays are negligible.

To demonstrate the stability of KALEN Human native LDL, material was stored at 4°C and then tested at intervals for protein concentration (figure below left) and relative electrophoretic mobility (REM) (figure below right). The results of these test methods demonstrate the stability of KALEN Human LDL for at least 8 weeks (protein concentration) and 10 weeks (REM).

Figure at left:
Protein concentration of a preparation of KALEN Human LDL. Values are plotted as a percentage of the mean.

Figure at right:
REM of a preparation of KALEN Human LDL stored at 4°C. Values are plotted as a percentage of the mean.