Kalen's Medium Oxidized Low Density Lipoprotein is prepared using strict manufacturing protocols and quality processes to provide a consistantly modified product that researchers worldwide rely on for their investigations.
Hunting for Human OxLDL?
KALEN Human Medium OxLDL is purified human LDL (d=1.019 - 1.063 g/mL),oxidized with copper (II)sulfate, purified, ultrafiltered and packaged aseptically under nitrogen. The product is provided in a solution with 154 mM NaCl and 0.34 uM EDTA,pH 7.2,at a protein concentration of 1 mg/mL. Purity is assessed by agarose gel electrophoresis.
| Catalog Number | Size | Price USD |
| 770202-7 | 1 mg | $290.00 |
| 770202-6 | 2 mg | $450.00 |
| 770202-4 | 5 mg | $795.00 |
KALEN Human MultiPoint Ox LDL Kit contains convenient, matched reagents to determine the dynamic range of your assay or provide the optimum oxidation level of Low Density Lipoprotein (LDL) for your research project. It is prepared from human LDL, oxidized under varying conditions to provide LDL with low (LOx), medium (MOx), and high (HOx) levels of oxidation.
The component with the medium level of oxidation (MOx) corresponds to KALEN Human OxLDL catalog number 770202.
Want more bars?
Each of these products is produced from human LDL (d 1.019 - 1.063) purified by progressive isopynic ultracentrifugation. This LDL is oxidized under different conditions resulting in three levels of oxidized products. An aliquot of native Low Density Lipoprotein is included in the kit as an appropriate control. Each of the products is provided in a solution with 154 mM NaCl and 0.34 uM EDTA, pH 7.2, at a protein concentration of 1 mg/mL.
| Catalog Number | Size | Price USD |
| 790202-7 | 4 x 1 mg | $790.00 |
| 790202-4 | 4 X 5 mg | $2200.00 |
Reproducibility of Oxidation
To evaluate the reproducibility of the lipoprotein modification, several preparations of Medium Oxidized Low Density Lipoprotein were assayed for thiobarbituric acid reactive substance (TBARS) and relative electrophoretic mobility (REM) as compared with native LDL. Values were determined for multiple preparations of KALEN Human OxLDL stored at 4°C.
Reproducibility of Oxidation
Several preparations of KALEN MultiPoint Oxidized Low Density Lipoprotein were evaluated for relative electrophoretic mobility (REM) as compared with native LDL. Electrophoretic migration on agarose gel is the parameter that best parallels the total extent of modification of the lipoprotein particle.
Changes in LDL during Oxidation
As a central component of atherosclerotic plaque formation, Oxidized Low Density Lipoprotein (Ox LDL) has been the subject of much research. LDL in the sub endothelial space of the arterial wall can become oxidized by interaction with the local monocytic cells, leading to inflammation and cytokine release, apoptosis and the development of foam cells and atherogenic plaques. The macrophages, endothelial cells and smooth muscle cells develop a fibrin cap which is susceptible to rupture through matrix metaloproteinases that are induced by oxidized LDL.
Lipoprotein Oxidation: Oxidation of Low Density Lipoprotein (LDL) causes changes in both the lipid and the protein moieties of the lipoprotein particle (see figure at right). Some of the polyunsaturated fatty acids are cleaved by oxidation to generate shorter chain and lower molecular weight components. A portion of these oxidized lipids and phospholipids can form adducts with protein; the protein moiety can also be broken down by oxidation.
Dr. Waxdal discusses some findings regarding the value of the TBARS assay and Oxidized-Low Density Lipoprotein (TBARS).
