Lipoprotein probes

Please contact Kalen for the availability of other fluorescent LDL probes as well as VLDL and HDL probes.



Kalen BioMedical

19640 Club House Road
Suite 415
Montgomery Village, MD USA 20886

Tel: 301.355.6155
Cell:301.908.8947
Fax: 301.355.6156

info@kalenbiomed.com

LDL probes

Purified human LDL (d=1.019-1.063), either native, acetylated, or oxidized, is reacted with a lipid stain, or a protein stain, for the production of the lipoprotein probes. The purified probes are ultrafiltered through a membrane, and packaged asceptically under nitrogen. The probes are provided in a solution with 154 mM NaCl and 0.34 uM EDTA, at pH 7.2. The protein concentration is 0.5 mg/mL. The purity of the probes is assessed by agarose gel electrophoresis.

DiI (3,3'-dioctadecylindocarbocyanine) is a fluorescent dye which permeates the lipid portion of the lipoprotein particle. These probes can be detected with a fluorescent microscope, fluorescent plate reader, or flow cytometer.

Biotin is a small molecule, covalently linked to the protein portion of the lipoprotein particle. A spacer group between the protein moiety of the lipoprotein and the biotin ensures its availability to aqueous solutions of avidin or streptavidin. Biotinylated materials are usually detected thru the secondary binding of an avidin or streptavidin conjugate.

More Information
on KALEN LDL probes:

Product Datasheets
Intended Use
Limited Use License

Parent Probe Catalog
Number
Size Price
USD
Native- LDL DiI-n-LDL 770230-9 0.5 mg $265.00
Acetylated -LDL DiI-Ac-LDL 770231-9 0.5 mg $295.00
Oxidized-LDL DiI-Ox-LDL 770232-9 0.5 mg $325.00
Native- LDL biot-n-LDL 770240-9 0.5 mg $265.00
Acetylated -LDL biot-Ac-LDL 770241-9 0.5 mg $295.00
Oxidized-LDL biot-Ox-LDL 770242-9 0.5 mg $325.00
Related Products:
LDL
Acetylated LDL
Oxidized LDL

       

©2007 Kalen BioMedical, LLC. All Rights Reserved